Caroteno-protein and exopolysaccharide production by co-cultures of Rhodotorula glutinis and Lactobacillus helveticus

J Ind Microbiol Biotechnol. 1997 Apr;18(4):272-7. doi: 10.1038/sj.jim.2900379.

Abstract

The lactose-negative yeast Rhodotorula glutinis 22P and the homofermentative lactic acid bacterium Lactobacillus helveticus 12A were cultured together in a cheese whey ultrafiltrate containing 42 g L-1 lactose. The chemical composition of the caroteno-protein has been determined. The carotenoid and protein contents are 248 micrograms g-1 dry cells and 48.2% dry weight. Carotenoids produced by Rhodotorula glutinis 22P have been identified as beta-carotene 15%, torulene 10%, and torularhodin 69%. After separating the cell mass from the microbial association, the exopolysaccharides synthesized by Rhodotorula glutinis 22P were isolated from the supernatant medium in a yield of 9.2 g L-1. The monosaccharide composition of the synthesized biopolymer was predominantly D-mannose (57.5%).

MeSH terms

  • Amino Acids / analysis
  • Carbohydrates / chemistry
  • Carotenoids / biosynthesis*
  • Culture Media
  • Fermentation
  • Fungal Proteins / biosynthesis*
  • Lactobacillus / metabolism*
  • Lactose / metabolism
  • Monosaccharides / analysis
  • Polysaccharides / biosynthesis*
  • Rhodotorula / metabolism*

Substances

  • Amino Acids
  • Carbohydrates
  • Culture Media
  • Fungal Proteins
  • Monosaccharides
  • Polysaccharides
  • Carotenoids
  • Lactose